| World Journal of Oncology, ISSN 1920-4531 print, 1920-454X online, Open Access |
| Article copyright, the authors; Journal compilation copyright, World J Oncol and Elmer Press Inc |
| Journal website http://www.wjon.org |
Original Article
Volume 2, Number 6, December 2011, pages 275-280
Accuracy of Cytology Specimen and Needle Core Biopsies for Detection of KRAS Mutation in Non-Small Cell Carcinoma: Comparison With Resection Specimen
Figure
Tables
| Name | Sequence |
|---|---|
| All Sequences Were Synthesized by Integrated DNA Technologies Inc. (Coralville, IA) | |
| *F for forward and R for reverse. | |
| *KRAS-ZuoF10 | 5'-TATAAACTTGTGGTAGTTGG-3' |
| *KRAS-ZuoR10 | 5'-ATTGTTGGATCATATTCGT-3' |
| Case ID | Biopsy | Surgical | Concordance | ||
|---|---|---|---|---|---|
| % tumor | Result (base) | % tumor | Result (base) | ||
| 1 | 30 | G12V (GTT) | 50 | G12V (GTT) | Yes |
| 2 | 25 | G12D (GAT) | 70 | G12D (GAT) | Yes |
| 3 | 40 | G12C (TGT) | 40 | G12C (TGT) | Yes |
| 4 | 30 | G12C (TGT) | 30 | G12C (TGT) | Yes |
| 5 | 30 | No mutant | 40 | No mutant | Yes |
| 6 | < 10 | G12S (AGT) | 70 | G12S (AGT) | Yes |
| 7 | 50 | No mutant | 60 | No mutant | Yes |
| 8 | 40 | G12A (GCT) | 30 | 12A (GCT) | Yes |
| 9 | 50 | No mutant | 60 | No mutant | Yes |
| 10 | 10 | G12A (GCT) | 25 | 12A (GCT) | Yes |
| Case ID | Cytology Cell Block | Surgical | Concordance | ||
|---|---|---|---|---|---|
| % tumor | Result (base) | % tumor | Result (base) | ||
| 1 | < 10 | G12V (GTT) | 50 | G12V (GTT) | Yes |
| 2 | 15 | G12C (TGT) | 40 | G12C (TGT) | Yes |
| 3 | 30 | G12D (GAT) | 60 | G12D (GAT) | Yes |
| 4 | 30 | G12V (GTT) | 60 | G12V (GTT) | Yes |
| 5 | 20 | No mutation detectable | 35 | G12C (TGT) | No |
| 6 | < 2 | No mutation detectable | 40 | G12C (TGT) | No |
| 7 | 2 | No mutation detectable | 20 | G12D (GAT) | No |
| 8 | 60 | No mutation | 60 | No mutation | Yes |
| Case ID | Cytology Smear | Surgical | Concordance | ||
|---|---|---|---|---|---|
| % tumor1 | Result (base) | % tumor | Result (base) | ||
| 1Not applicable since the tumor cells are isolated on smears with pinpoint technique; 2Small mutation peak at code 13 (GAC) as well | |||||
| 1 | N/A | No mutant | 30 | No mutant | Yes |
| 2 | N/A | G12D (GAT) | 60 | G12D (GAT) | Yes |
| 3 | N/A | G12D (GAT) | 30 | G12D (GAT) | Yes |
| 4 | N/A | G12V (GTT) | 25 | G12V (GTT) | Yes |
| 5 | N/A | G12C (TGT) | 35 | G12C (TGT) | Yes |
| 6 | N/A | G12C (TGT) | 40 | G12C (TGT) | Yes |
| 7 | N/A | G12D (GAT)2 | 20 | G12D (GAT) | Yes |
| 8 | N/A | G12D ( GAT) | 35 | G12D ( GAT) | Yes |
| 9 | N/A | G12F (TTT) | 40 | No mutation | No |