Hyaluronan (HA) and H11 Antigen (HABP) Expression in Cervical Cancer Sections and Their Significances as Prominent Markers

Abstract

This article has been retracted.

Background: Cancer is a disease characterized by a population of cells that grow and divide without respect to normal limits, invade and destroy adjacent tissues, and may spread to distant anatomic sites through a process called metastasis. These malignant properties of cancers differentiate them from benign tumors, which are self-limited in their growth and do not invade or metastasize for the development and progression of tumor cells. Cancer is a result of multistep process involving accumulation of genetic alternation, which results in loss of cell-cell interaction, increasing invasive migration, loss of control in cell division and abnormal matrix assembly. Carcinoma of the cervix is one of the most common malignancies. Cervical differentiation requires changes in the composition and structure of extracellular matrix. However, the biological roles of Hyaluronan (HA) in cervical remodeling are still under investigation and HA-receptors are not studied during cervical pathological remodeling. Hence, in the current study we investigated the expression and association of extracellular matrix components namely hyaluronan-hyaluronic acid binding protein (H₁₁ antigen) (HA-HABP) in normal and invasive lesion in cervical tissue section slides.

Methods and Results: Cervical tissues from normal and suspected cancer patients from hospitals were examined. Immunohistochemistry was performed with specific HA probe, b. PG (biotinylated proteoglycan) and anti-HABP antibody H₁₁B₂C₂ for H₁₁ antigen. HA and H₁₁ antigen are overexpressed in well-differentiated tumors during progression.

Conclusions: We conclude that the expression of HA and H₁₁ antigen and their interaction are very important in identifying cervical cancers and probably H₁₁ antigen can be used as a biomarker in identifying the tumor cells in cervical lesions.




doi:10.4021/wjon287e