The Vascularised Groin Chamber: A Novel Model for Growing Primary Human Liposarcoma in Nude Mice

Daniel Johannes Tilkorn, Sammy Al-Benna, Joerg Hauser, Andrej Ring, Lars Steinstraesser, Adrien Daigeler, Inge Schmitz, Hans Ulrich Steinau, Ingo Stricker

Abstract


Background: The preclinical development of anti-sarcoma drugs has been primarily based on the subcutaneous transplantation of xenografts. Transplant survival remains an obstacle of current models which has been attributed to the period of hypoxia after transplantation. We hypothesized that primary soft tissue sarcoma models with an intrinsic tissue engineered vascular supply would be easily reproducible. The aim of this study was to establish a model of primary human soft tissue sarcoma with an intrinsic vascular supply.

Methods: Primary soft tissue sarcoma cells from resected human liposarcomas isolated and divided into tumour fragments were transplanted into a silicon chamber, placed around the superficial epigastric vessels in mice. Sarcoma xenograft samples were analysed histomorphologically (light/electron microscopy and immunohistochemistry).

Results: All primary soft tissue sarcoma transplants engrafted, leading to solid tumours within 3 weeks. Histological and immunohistochemical staining conï¬rmed the mouse xenografts as identical high grade liposarcomas compared to original tumour tissue.

Conclusion: This study established a reproducible xenograft model of primary human liposarcoma. This animal model could be of high value for studying human soft tissue sarcomas and their therapy.




doi:10.4021/wjon496w


Keywords


Angiogenesis; Soft tissue sarcoma; Tissue engineering; Xenograft

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